Protocols have been used by cail.cn
This section is generated from parts of my dissertation, including all the experimental protocols I used in Bear Lab - UNC. If you have questions about these protocols, please feel free to email me. Thanks!The yellow highlighted part is the correction to the original PDF file.
A.5 TaqMan qRT-PCR
A.11 Actin TIRF assay
A.18 Peptide mapping
A.27 Single cell tracking
A.28 Kymography analysisIn step one, for each 800 ml Buffer, add 0.264g MgCl2-6H2O and 0.166g NaH2PO4-H2O
B.26 Freeze E.coli cellsThe dish used for transfection is 60mm x 15mm style (for example: Polystyrene cell culture dish from Corning, 430166). Serum starvation is overnight.
B.31 DSP cross-linkingBacto ™ has a product called Terrific Broth (0438-17-0), which has additional KH2PO4 9.4g and K2HPO4 2.2g for each liter.